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Centrifuge cleaning

Nick_Devlin_239717

Hi everybody - this is my first post, so go easy.

I recently picked up a good deal on a secondhand centrifuge (IEC Centra MP4R spinning bucket (4 x 250ml)) and I'm working though my ideas to clean it thoroughly before use. I was told by the seller (a dealer for disposal of used equipment) that it had a certificate detailing full decontamination with IPA (iso-propyl alcohol I assume) but I have no point of reference as to the thoroughness or adequacy of this. For my purposes, I'm going to assume it is not adequate enough, but I don't know by how much. For what it is worth, I intend to only spin in bottles, although the bottles for this model are no longer available, but some of the Thermo Scientific look like they will fit snugly. In an ideal world, this is my gateway centrifuge until the Booker and Dax model is available.

Anyway, I wanted to know if anyone had thoughts on the best way to clean it. I'm thinking a combination of cleaning with bleach, ethanol and pressure cooking the rotor and buckets and then maybe doing it all again several times. I'm aware that bleach can corrode the metal components - but I'm unclear if the issue is related to a single bleaching or repeated occasions.

Any help would be greatly appreciated.

best

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Comments

lfmichaud

Nice centrifuge! I was in the same situation as you and did many scrubing of the entire interior. used soap alcool and soap. I did the same time of treatment with the buckets plus a couple of hours of pressure cooker. I'm not really concerned about what's left as I never put anything directly in the bucket. To be thorough I should get a geiger counter but the seller wasn't from North Korea or Iran so I should be safe ;-)

Brandon_Byrd_40557

Dave Arnold recommends soaking the buckets in bleach overnight and then pressure cooking them. If you have access to an autoclave, that would work even better. Clean the interior and exterior with a concentrated bleach solution several times (and let it air dry in between).